细胞-基质粘附实验
关键词: 细胞-基质 粘附实验来源: 互联网
【求助】有没有高人做过细胞-基质粘附实验啊?
参与者:从天而降的少女
96孔扳上是先铺mtrix gel 再铺FN,还是直接铺FN就可以了呢?滴上去以后风干了就可以吗?还是要等一段时间才可以加细胞悬液?谁告诉我一下啊,多谢.我想检测肿瘤细胞的黏附能力.
有没有高人做过细胞-基质粘附实验啊?
参与者:sunnyziyang
直接铺FN,超净台内过夜风干,使用前PBS 冲洗2遍,重新水化,再加1%BSA100μl/孔封闭,培养箱内培养1h后加100μl细胞悬液
参与者:xinzhijinbi
请问在材料上种细胞时测细胞在材料上的黏附力能这样测吗?
具体咋做呢?
参与者:hanhailanbo
现在有现成的试剂盒检测细胞的粘附能力,美国cell biolabs公司的试剂盒不错,好像国内北京西美杰有代理。
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