Isolation of a Membrane-Bound Enzyme, 5-Nucleotidase
Membrane enzymes may be peripheral or integral proteins. The attachment of peripheral enzymes is mediated mainly by association with other membrane proteins and consists primarily of hydrophilic, electrostatic interactions. Integral membrane proteins are primarily bound by hydrophobic interactions with the lipid bilayer, either via one or more transmembrane peptide domains (1 ) or by possession of a glycosyl-phosphatidylinositol (GPI) membrane anchor at the carboxy-terminus (2 ). The purification of a membrane enzyme thus requires two additional considerations compared to the isolation of a cytosolic enzyme: the isolation of a membrane fraction containing the enzyme and the solubilization of the enzyme from this fraction. Once solubilization has been achieved and maintained, the enzyme can be treated essentially as a soluble enzyme and subjected to a wide range of purification techniques.
- Large Scale Production of Nuclear Receptor Ligand-Binding Domains
- Isolation and Culture of Porcine Embryonic Stem Cells
- Zymographical Techniques for Detectionof Matrix Metalloproteinases
- 抑瘤粉抗肝部细胞实验研究
- Fluorescence Immunohistochemistry in Combination with Differential Interference Contrast Microscopy for Studies of Semi-ultrathi
- In Vitro Maturation (IVM) of Porcine Oocytes
- 质体醌(plastoquinone, PQ)
- 心肌细胞培养方法详述
- Derivation of Human Embryonic Stem Cells in Xeno-Free Conditions
- Transwell侵袭实验总结 -Transwell的其他应用的实验步骤