细胞免疫荧光的操作步骤
关键词: 细胞 免疫荧光来源: 互联网
相关专题
1.细胞爬片;
2.4%多聚甲醛固定10min(固定细胞器 用预冷的70%甲醇+30%丙酮);
3.PBS 漂洗5min;
4.0.5% Triton 穿孔15min(丙酮固定法不用透化处理);
5.PBS 漂洗2次,每次5min;
6.1%BSA 封闭30min;
7.加入1%BSA 稀释的一抗,于37℃杂交2h;
8.PBS漂洗2次,每次5min;
9.加入1%BSA稀释的二抗,于37℃杂交1h;
10.PBS漂洗2次,每次5min;
11.5ug/ml DAPI染色2min;
12.抗淬灭封片剂封片。
抗淬灭封片剂:
2.5% DABCO (w/v)
50mM Tris(pH8.0)
90%甘油
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