转染过程
关键词: 来源: 互联网
问:
哪位高手给分别指点一下脂质体和质粒转染的具体过程!
答:
具体过程都大至差不多,一管混匀质粒,另一管混匀脂质体,脂质体混匀后过几分钟,逐滴加入混匀的脂质体,温柔混匀。但是我想更为重要的有二个方面,一是你用的哪一公司的脂质体,然后查阅其产品说明书,可能不同的公司的脂质体所用的溶剂是不一样的,具体按说明书进行操作;第二是查阅你所要转染细胞的转染密度与大致转染效率如何,便于以后分析问题。
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