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转染细胞的问题
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问：我想问个问题关于转染细胞的质粒，必须用试剂盒提取的么？用碱裂解法可以么？抽提两次浓缩一次，测得浓度是8点多，ratio是1.814，可以送去转染细胞不？人的细胞系。
答：碱裂解法比试剂盒提取的纯度要低，但是浓度高。其实，剂盒提取的原理和碱裂解法的一样，只不过最后过个吸附柱和蛋白洗脱，从而纯度较好。碱裂解法提取可以用来转染。几年前我还没用盒子时都是用碱裂解法提取转染，效果ok。
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