The Cobblestone-Area-Forming Cell Assay
Hematopoietic stem cell (HSC) subsets are defined by the capacity to which their offspring can contribute to the various mature blood-cell lineages. However, the proliferative potential of stem cells is highly dependent on the environment in which they reside, and it is only in retrospect that the characteristics of a stem cell can be identified. Partly because of these elusive properties of stem cells, various functional assays to measure their frequency have been developed, both in vivo and in vitro. Various chapters in this volume each describe one of these assays. All these assays have the ability to quantify stem cells with certain properties, but not others. Therefore, the determination of which stem-cell assay will be most appropriate to use is highly dependent on a particular experimental setting. This chapter describes the cobblestone-areaforming cell (CAFC) assay (1).
- Automated Live Microscopy to Study Mitotic Gene Function in Fluorescent Reporter Cell Lines
- Detection of Herpes Simplex Virus Dependent Apoptosis
- Whole-Mount Assays for Gene Induction and Barrier Formation in the Developing Epidermis
- 大鼠胆固醇(CH)酶联免疫分析
- Functional Analysis of Nocturnin: A Circadian Clock-Regulated Gene Identified by Differential Display
- Therapeutic Inhibition of Angiogenesis
- Quantitative In Situ Hybridization of Tissue Microarrays
- The Measurement of Nitric Oxide and Its Metabolites in Biological Samples by Ozone-Based Chemiluminescence
- Assays to Monitor Degradation of the EGF Receptor
- Testing for Mycoplasma by Indirect DNA Stain (Hoechst 33258 stain)