p53-Induced Gene Expression Analysis
Researchers in the p53 field have successfully used many high-throughput screening technologies to analyze and characterize p53 -induced gene expression. This chapter will focus on one such technology, the Affymetrix GeneChip�. DNA-Chip technology has grown rapidly over the last several years. The ability to hybridize RNA from a sample to thousands to tens of thousands of known and unknown cDNAs spotted on a microarray chip has led to the explosion of information ranging from macro-global expression pattern changes to micro-gene-specific expression changes. The relative ease of making a nonradioactive probe from either total RNA or mRNA to be hybridized to a GeneChip makes microarray technology highly attractive.
- Laser-Assisted Microdissection and Isolation of DNA and RNA
- Flow Cytometric Methods for Detection and Quantification of Apoptosis
- Lung-Specific Expression of Mutant p53 as Mouse Model for Lung Cancer
- Development of Orthotopic Pancreatic Tumor Mouse Models
- Differential Screening of cDNA Libraries for Analysis of Gene Expression During Tumor Progression
- Identification and Characterization of Hematopoietic Stem and Progenitor Cell Populations in Mouse Bone Marrow by Flow Cytometry
- The Use of Denaturing High Performance Liquid Chromatography (DHPLC) for Mutation Scanning of Hereditary Cancer Genes
- 如何检测不同细胞siRNA转染效率
- Classification of AML Using a Monoclonal Antibody Microarray
- 真核生物翻译的调控