Isolation and Culture of Human Macrophages
Methods to isolate and culture human monocyte-derived macrophages and alveolar macrophages are described. Monocytes are obtained from buffy-coat preparations by Ficoll density gradient centrifugation, followed by adhesion-mediated purification on tissue culture or gelatin-coated plastic. The monocytes differentiate into macrophages in vitro by culturing in medium containing autologous human fibrin-depleted plasma. Alveolar macrophages can be purified from bronchoalveolar fluid samples by adhesion to tissue culture plastic. If resected lung tissue is available, alveolar macrophages can be obtained by mechanically disrupting the lung parenchyma, followed by adhesion-mediated purification.
- Application of Cre/loxP in Drosophila: Site-Specific Recombination and Transgene Coplacement
- Isolation and Culture of Human Umbilical Vein Endothelial Cells
- A Novel Technical Approach for the Measurement of Individual ACAT-1 and ACAT-2 Enzymatic Activity in the Testis
- Analysis of Molecular Chaperone Activities Using In Vitro and In Vivo Approaches
- Measurement of Phospholipase C Activity in Brain Membranes
- Derivation of Human Embryonic Stem Cells in Xeno-Free Conditions
- 细胞培养器械的清洗与消毒方法
- 小鼠白细胞介素1(IL-1)酶联免疫分析(ELISA)
- 猪白细胞介素-12(IL-12)酶联免疫分析(ELISA)
- Sperm Chromatin Structure Assay (SCSA)