sf9,空斑试验,滴度测定
关键词: sf9 空斑试验 滴度测定来源: 互联网
【求助】sf9,空斑试验,滴度测定
参与者:xiaoniziwendeng
我用的是bac-to-bac系统表达的目的蛋白。加上荧光底物后,96孔板读数,野生病毒和目的病毒相差不大,而且细胞感染后脱落严重,我该如何做呢?很希望能得到大家的帮助
参与者:yangea
以下问题请参考排除:
1、是挑取的单空斑进行病毒扩增的吗?
2、荧光底物是否摸索了浓度梯度,即是否找到了作用最为明显的使用浓度;
3、细胞感染后脱落严重说明使用的病毒滴度高了,或者在实验时手法太重,比如接种细胞、加病毒混匀操作等。
多多交流!
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