DNA 标记（主要内容如下）

•   DNA Labeling by Nick Translation
•   Random Primed Labeling
•   End-Labeling
•   Purification of Labeled DNA
•   Non-isotopic Labeling
•   Others

DNA Labeling by Nick Translation

§         DNA Labeling by Nick Translation (Ambros Lab)

§         Nick Translation (Worthington)

§         Nick Translation

§         Nick Translation (Promega)

§         Nick Translation (Life Technologies)

Random Primed Labeling

・         Random Primer Labeling (Karl Clark) Prepare radioactively labeled DNA strands for the detection of target DNA or RNA sequences in usr/localious applications including Southern and northern blot hybridization. Detailed protocol for labeling, purification and determination of incorporation

・         Random Primed Labelling with P32 (Molecular Genetics Network Lab)

・         Random Primer Labeling (Worthington)

・         Random Primer Labeling (Crawford Lab)

・         Random primer labeling (Amersham Pharmacia)

・         Random Primer  DNA Labeling (Life Technologies)

・         Random Primer DNA labeling (Fermentas) Labeling of DNA by random oligonucleotide-primed synthesis

・         Random Hexamer Labeling of cDNA Probes (PMCI Research)

End-Labeling

・         5'-End Oligo Fluorescence Labeling (Amersham Pharmacia) Labeling of  oligonucleotides with a single fluorescein residue at the 5' end

・         5' end labeling of oligonucleotides with gamma-32 P-ATP (Petra Klaff) Labeling and purification method in great detail

・         3'-end Labeling (Promega)

・         DNA Labeling using Klenow Fragment I (Lazo Lab)

・         End-labeling of DNA fragments Single end or double ends, can also be used for RNA

・         Labeling of Oligonucleotides (PMCI Research) Label oligo with polynucleotide kinase. 

・         Alkaline phosphatase labeling of oligos (Promega) Provides useful guide for preparing phosphatase conjugated oligos

・         Kinase End-Labeling of DNA (Roe, OU)

・         Endlabeling of DNA fragments with Klenow polymerase (Molecular Genetics Network Lab)

・         5'-end Labeling (Amersham Pharmacia)

・         Fill in Labeling of DNA (Mike A. Dyer)

・         5'-End Labeling (Fermentas) T4 polynucleotide kinase  labeling (ds and ss DNA or RNA; protruding, recessed and blunt 5'-ends) for (1)end labeling of DNA or RNA probes in DNA hybridization and DNA footprinting experiments, etc; (2)obtaining labeled molecular weight markers;(3)obtaining labeled molecular weight markers; (4)labelling of primers for sequencing; (5)addition of 5'-phosphates to oligonucleotides to allow subsequent ligation.

・         Oligonucleotide labeling with 32 P (Hahn Lab) T4 polynucleotide kinase labeling 

Purification of Labeled DNA

§         5' end labeling of oligonucleotides and purification (Petra Klaff) Labeling and purification method (ethanol and CTAB in great detail

§         Oligo Purification on Acrylamide Gels (Botwell Lab) Purification of labeled oligos

・         Sephadex G-50 Spin Column (gopher://iubio.bio.indiana.edu/ ) For purifying labeled probe

・         Spin Column chromatography (Schimenti Lab) Used to removed unincorporated nucleotides from labeling reactions.

・         Oligonucleotide Purification by PAGE and DEAE (Hahn Lab)

・         PAGE Purification of Oligonucleotides (Life Technologies) Protocol for PAGE Purification of Oligonucleotides Protocol for PAGE Purification of Oligonucleotides Oligonucleotides contaminated by significant amounts of aborted synthesis products (e.g., n-1,...

Non-isotopic Labeling

・         PCR DNA Biotinylation (KPL) A rapid method for biotinylating DNA probes through incorporation of biotin-N4 -dCTP via a thermostable DNA polymerase in the polymerase chain reaction

・         Determination of efficiency of digoxigenin labelling (Molecular Genetics Network Lab)

・         Non-isotopic Labeling (Gottschling Lab) Protocols for preparing DNA, RNA probe and molecular marker

Others

・         PCR Products Labeling (Amersham) Guidelines for preparation of radioactively-labeled PCR products for use as probes