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Rapid Isolation of Genomic DNA from Human Oral Mucosa
关键词： rapid isolation来源： 互联网
- Take an oral smear
- Dissolve in 50 ml H2 O through vigorous shaking
- Pellet cells 5 min at 4000 rpm
- Re-suspended the pellet in 400 μl Lysis buffer (50 mM TRIS-Cl pH 8, 10 mM EDTA, 2% SDS)
- Incubate for 5 min at 65¡ãC
- Add 250 μl 4.5 M NaCl and mix
- Pellet cellular debris (13000 rpm for 4 min). Genomic DNA stays in the supernatant
- Precipitate DNA by adding 650 μl of isopropanol to the supernatant and centrifuge 5 min at 13000 rpm
- Re-suspend the DNA in an appropriate buffer (e.g. TrisCl, pH=8.0)
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