Composition per liter:

Agar ……15.0g

Tryptone……10.0g

Peptone ……5.0g

Yeast extract ……1.0g

Synthetic seawater …… 1.0L

pH 7.8 ± 0.2 at 25°C

Synthetic Seawater Composition per liter:

NaCl ……24.0g

MgCl2·6H2O ……11.0g

Na2SO4……4.0g

CaCl2·6H2O……2.0g

KCl……0.7g

KBr……0.1g

SrCl2·6H2O ……0.04g

H3BO3 ……0.03g

NaSiO3·9H2O……5.0mg

NaF……3.0mg

NH4NO3 ……2.0mg

Fe3PO4·4H2O ……1.0mg

Preparation of Synthetic Seawater: Add components to distilled water and bring volume to 1.0L.

Mix thoroughly.

Preparation of Medium: Add agar, tryptone, peptone, and yeast extract to synthetic seawater and bring volume to 1.0L. Mix thoroughly. Adjust pH to 7.8. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure –121°C. Pour into sterile Petri dishes or leave in tubes.

Use: For the cultivation and maintenance of Halobacillus halophilus, Halomonas spp., Vibrio harveyi,Cobetia marina, and Ruegeria atlantica.