1.Yeast (93.75 g) + Super Q Water (562.5 ml) per flask.

2.Place in incubator at 37°C for 20 minutes.

3.Heat to 90-100°C for 5 minutes.

4.Cool on ice.

5.Pour into centrifuge bottles and centrifuge at 2500 rpms for 30 minutes (4°C).

6.Filter supernatant through glass pads twice.

7.Measure into bottles and label.

8.Autoclave for 2-5 minutes at 115°C.

9.Freeze in 45 and 90 ml aliquots.

125 g yeast per 750 ml water = 1 batch

Yield is approximately 700 ml.