| AG1 endA1 recA1 gyrA96 thi-1 relA1 glnV44 hsdR17(rK - mK + ) AB1157 thr-1, araC14, leuB6(Am), Δ(gpt-proA)62, lacY1, tsx-33, qsr'-0, glnV44(AS), galK2(Oc), LAM-, Rac-0, hisG4(Oc), rfbC1, mgl-51, rpoS396(Am), rpsL31(strR), kdgK51, xylA5, mtl-1, argE3(Oc), thi-1 - Bachmann BJ: Derivation and genotypes of some mutant derivatives of Escherichia coli K-12.
Escherichia coli and Salmonella typhimurium. Cellular and Molecular Biology (Edited by: F C Neidhardt J L Ingraham KB Low B Magasanik M Schaechter H E Umbarger). Washington, D.C., American Society for Microbiology 1987, 2:1190-1219. See CGSC#1157 BL21 E. coli B F- dcm ompT hsdS (rB - mB -) gal [malB+ ]K-12 (λS ) -
- The "malB region" was transduced in from the K-12 strain W3110 to make the strain Mal+ λS . See Studier et al. (2009) J. Mol. Biol. 394(4), 653 for a discussion of the extent of the transfer.
- Stratagene E. coli Genotype Strains
BL21(AI) F– ompT gal dcm lon hsdSB (rB - mB - ) araB::T7RNAP-tetA -
- an E. coli B strain carrying the T7 RNA polymerase gene in the araB locus of the araBAD operonq .
- Transformed plasmids containing T7 promoter driven expression are repressed until L-arabinose induction of T7 RNA polymerase.
- Derived from BL21.
- product page for more information.
BL21(DE3) F– ompT gal dcm lon hsdSB (rB - mB - ) λ(DE3 [lacI lacUV5-T7 gene 1 ind1 sam7 nin5]) -
- an E. coli B strain with DE3, a λ prophage carrying the T7 RNA polymerase gene and lacIq
- Transformed plasmids containing T7 promoter driven expression are repressed until IPTG induction of T7 RNA polymerase from a lac promoter.
- Wood, 1966 ) by transducing to Met+ .
- See the original Studier paper or the summary in Methods in Enzymology for more details.
BL21 (DE3) pLysS F- ompT gal dcm lon hsdSB (rB - mB - ) λ(DE3) pLysS(cmR ) - pLysS plasmid chloramphenicol resistant; grow with chloramphenicol to retain plasmid
- Chloramphenicol resistant
- The pLysS plasmid encodes T7 phage lysozyme, an inhibitor for T7 polymerase which reduces and almost eliminates expression from transformed T7 promoter containing plasmids when not induced.
- see Moffatt87 for details of pLysS and pLysE plasmids
BNN93 F- tonA21 thi-1 thr-1 leuB6 lacY1 glnV44 rfbC1 fhuA1 mcrB e14-(mcrA- ) hsdR(rK - mK + ) λ- (责任编辑：大汉昆仑王) |
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