苯丙氨酸培养基
关键词: 苯丙氨酸培养基来源: 互联网
成分
酵母浸膏 3g
DI-苯丙氨酸(或L-苯丙氨酸1g) 2g
磷酸氢二钠 1g
氯化钠 5g
琼脂 12g
蒸馏水 1000mL
制法
加热溶解后分装试管,121℃高压灭菌15min,使成斜面。
试验方法
自琼脂斜面上挑取大量培养物,移种于苯丙氨酸琼脂,在36±1℃培养4h或18~24h。滴加10%三氯化铁溶液2~3滴,自斜面培养物上流下,苯丙氨酸脱氨酶阳性者呈深绿色。
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