Enzyme-Linked Immunosorbent Assays
Sensitive immunoassays were developed in the 1950s and 1960s using radioactive isotopes. The application of enzymes as labels (1 ,2 ) in the late 1960s increased the potential sensitivity and safety of immunoassays. The widespread popularity of enzyme-linked immunosorbent assays (ELISAs) owes much to the use of microtiter plates (3 ). It was probably the adoption of microtiter plates that provided ELISA with its greatest advantag-convenience. However, the convenience of plastic microtiter plates carries with it a limitation, the ease with which reagents bind to plastic and the way in which they perform once bound. In this chapter, I will review ELISA procedures and discuss their advantages and disadvantages.
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- 胰蛋白胨与蛋白胨的区别
- 蛋白胨水制备(靛基质试验用)
- 打印病
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- In Vitro and In Vivo Interactions of Nonpathogenic Bacteria With Immunocompetent Cells
- Assaying Interleukins in Plasma in the Course of Acute Myocardial Infarction
- Identification of Novel Pathogenicity Genes by PCR Signature-Tagged Mutagenesis and Related Technologies
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