Measuring Cheating, Fitness, and Segregation in Dictyostelium discoideum
Dictyostelium has become a model organism for the study of social evolution because of the stage in its life cycle where thousands of independent amoebae together form a fruiting body. Some individuals die to form a stalk that holds aloft the remaining cells for dispersal to new environments as spores. Different genotypes can aggregate together, creating opportunities for exploitation by cheaters that contribute a smaller proportion of cells to the stalk. Clustering of genotypes into separate fruiting bodies reduces the opportunities for cheating. Some genotypes achieve this by segregating after aggregation. Here we describe techniques for assaying cheating and segregation in D . discoideum . We cover how to grow and maintain cells, fluorescently label genotypes, design experiments for accuracy and precision, calculate fitness and segregation, and interpret the results.
- 大肠杆菌培养方法
- 土壤微生物学
- 常用固体培养基的质量控制标准
- 花鳗
- 34 Expression of scFvs and scFv Fusion Proteins in Eukaryotic Cells
- Immunoblotting and Enzyme-Linked Immunosorbent Assay
- Isolation of Dictyostelium Nuclei for Light and Electron Microscopy
- Proteomic Analysis of Proteins Secreted by Streptococcus pyogenes
- In Vitro Peptidoglycan Synthesis Assay with Lipid II Substrate
- Genetic Manipulation of the Obligate Chemolithoautotrophic Bacterium Thiobacillus denitrificans