Media and Growth Conditions for Induction of Secondary Metabolite Production
Growth media and incubation conditions have a very strong influence of secondary metabolite production. There is no consensus on which media are the optimal for metabolite production, but a series of useful and effective media and incubation conditions have been listed here. Chemically well-defined media are suited for biochemical studies, but in order to get chemical diversity expressed in filamentous fungi, sources rich in amino acids, vitamins, and trace metals have to be added, such as yeast extract and oatmeal. A battery of solid agar media is recommended for exploration of chemical diversity as agar plug samples are easily analyzed to get an optimal representation of the qualitative secondary metabolome. Standard incubation for a week at 25�C in darkness is recommended, but optimal conditions have to be modified depending on the ecology and physiology of different filamentous fungi.
- 紫外线灭菌相关问题总结
- 肠道杆菌
- 猫鲨
- 绿脓杆菌外毒素A
- 细菌的菌落特征与繁殖
- Determination of Pyrethroids in Blood Plasma and Pyrethroid/Pyrethrin Metabolites in Urine by Gas Chromatography-Mass Spectromet
- 咸黄鱼
- Analysis of Linker Histone Binding to Mono- and Dinucleosomes
- Use of Genome-Scale Metabolic Models in Evolutionary Systems Biology
- Isolation, Characterization, and Diversity of Novel Radiotolerant Carotenoid-Producing Bacteria