Construction of Carotenoid Biosynthetic Pathways Through Chromosomal Integration in Methane-Utilizing Bacterium Methylomonas sp.
Methylomonas sp. strain 16a is an obligate methanotrophic bacterium that uses methane or methanol as the sole energy and carbon source. In order to engineer a stable strain to produce carotenoids, integration of genes or gene clusters in various nonessential locations in the chromosome is used. Construction of a canthaxanthin-producing strain involves the integration of canthaxanthin biosynthetic genes including the crtW gene for the β-carotenoid ketolase. Addition of the crtZ gene that encodes the β-carotenoid hydroxylase in this strain leads to the production of astaxanthin. Further increase in titer and yield for astaxanthin is obtained by integration of another set of astaxanthin biosynthetic gene cluster in a separate location of the chromosome.
- 微生物鉴定中常用的生化反应
- 蛋白胨水制备(靛基质试验用)
- 白粉病菌类(pathogens causing;powderymildew)
- 发酵罐的清洗的注意事项
- Characterization of HLA-DQ-Specific Peptide-Binding Motifs
- APEX DNA Microarray for the Identification of Pathogenic Fungi
- Retrofitting BACs and PACs With LoxP Transposons to Generate Nested Deletions
- Plesiomonas shigelloides: Detection by PCR
- Small-Scale Protein Production With the Baculovirus Expression Vector System
- The Genetic Basis of Microbial Resistance to Antimicrobial Peptides