Basic Investigations in Saccharomyces cerevisiae
This chapter aims to provide the reader with a one-stop reference to the basic procedures needed to grow, store, mate, and sporulate yeast cells. Starting with recipes for the different types of media, the chapter then goes on to explain how cells are grown to the appropriate cell numbers at the correct stage in the growth cycle. It also provides a detailed explanation on both short- and long-term storage of yeast cells. It then explains how to set up genetic crosses, before finally dealing in some detail with the demanding technique of diploid cell sporulation and spore isolation. It ends with an introduction to the Internet-based yeast resources, which are becoming increasingly important in the investigation of Saccharomyces cerevisiae in the post-genomic era.
- 细菌感染的诊断和特异防治
- Adenovirus Protease
- 澳洲银鲈
- The Direct Epifluorescent Filter Technique (DEFT)
- Simultaneous Detection of RNA and Proteins in Adenovirus-Infected Cells by Fluorescence In Situ Hybridization (FISH) and lmmunos
- Targeted Isolation of Proteins from Natural Microbial Communities Living in an Extreme Environment
- Measuring Oxidative mtDNA Damage and Repair Using Quantitative PCR
- Interleukins in Graves Disease
- Linkage and the Transmission Disequilibrium Test in Complex Traits: Celiac Disease as a Case Study
- Animal Models of Helicobacter-Induced Disease: Methods to Successfully Infect the Mouse