Use of NMR to Study H. pylori Metabolism
Until recently, the culture of Helicobacter pylori in vitro has only been achievable with complex undefined media supplemented with blood products, such as Brain-heart infusion (BHI) medium, blood agar, chocolate agar, or lysed blood agar (1 ). Substrate utilization and metabolic activity during growth on these media is difficult to define; this has led to the use of techniques such as nuclear magnetic resonance (NMR) spectroscopy to investigate the metabolic pathways active in H. pylori (e.g., refs. 2–5). Defined media for the growth of H. pylori have now been described (6 ,7 ), however, and may facilitate future metabolic studies in this organism by more conventional techniques.
- 菌种保藏方法大全
- 酵母菌(yeast)
- 显性感染(Apparent infection)
- Streptomyces:Protocols/Mini-Maxi Prep
- Preparation of Bacteriophage Lysates and Pure DNA
- Evolutionary Aspects of a Genetic Network: Studying the Lactose/Galactose Regulon of Kluyveromyces lactis
- Isolation and Quantification of Genomic DNA from Mycobacterium tuberculosis
- Proteomic Analysis of Proteins Secreted by Streptococcus pyogenes
- Propagation and Assay of the JC Virus
- Chemical Mutagenesis of Mycobacteria