Identification of Mycoplasmas by Dot Immunobinding on Membrane Filtration (MF Dot)
The definitive identification of mycoplasmas is usually based on serological procedures, including growth inhibition (1 ,2 ), metabolic inhibition (3 ,4 ), immunofluorescence (5 –7 ), and immunobinding assays (8 –14 ). The technique most commonly adopted for the routine identification of mycoplasma species isolated from clinical material is, at present, the immunobinding assay involving either broth culture (12 ,14 ), mycoplasma colonies on agar plates, or the imprints of colonies (8 –13 ). Several procedures with polyclonal or monoclonal antibodies (MAb) have been described. All these assay systems are based on the detection of mycoplasma surface antigens, which are believed to be highly specific.
- 发酵乳酸饮料的制作
- 酵母培养的注意事项
- 自生固氮菌(Azotobacteria)
- Two-Dimensional Gel Electrophoresis-Based Proteomics of Mycobacteria
- Selection of Antibodies Able to Rapidly Enter Mammalian Cells
- Analysis of Lipid Biosynthesis and Location
- Detection of Human Caliciviruses and Astroviruses in Stools by RT-PCR
- Construction of Recombinant Vaccinia Viruses Using Leporipoxvirus-Catalyzed Recombination and Reactivation of Orthopoxvirus DNA
- Inducible Expression Systems for Mycobacteria
- Protein Turnover Methods in Single-Celled Organisms: Dynamic SILAC