Purification and Characterization of MHC Class II Containing Organelles in Mouse Bone-Marrow-Derived Dendritic Cells
The initiation and propagation of the immune responses is dependent on the ability of antigen-presenting cells (APC) to convert proteins into peptides, to load them intracellularly onto major histocompatibility complex (MHC), and then to deliver the peptide-MHC complexes to the plasma membrane in order to stimulate T cells. It is becoming increasingly clear that most of the intracellular compartments that contain MHC class II products in APC simply represent the conventional endosomes and lysosomes that are expressed in all cell types (1 ). However, data from cell-fractionation studies, predominantly those using electrophoresis techniques, show that in professional APC, a class of class II-containing endosomes that is specialized for antigen processing may exist. Strong support for this possibility comes from the observation that such specialized structures, designated CIIV, are particularly abundant in mature dendritic cells (DC; ref 2 ).
- 幽门螺旋杆菌的特征、培养及诊断
- PDA培养基,牛肉膏蛋白胨培养基的配方
- 病毒感染检查方法(形态学观察与免疫学诊断)
- 打粉病
- Histologic Scoring of Gastritis and Gastric Cancer in Mouse Models
- Transfection Complexes Generated with Adenovirus and Polyethylenimine-Condensed DNA
- Analysis of Herbicides in Water by On-Line In-Tube Solid-Phase Microextraction Coupled With Liquid Chromatography-Mass Spectrome
- Microarrays for Bacterial Typing: Realistic Hope or Holy Grail
- Evaluation of Cytokines in Migraine Patients by ELISA
- Microbial Proteases