An Array Biosensor for Detection of Bacterial and Toxic Contaminants of Foods
The Naval Research Laboratory has developed an array-based biosensor system capable of detecting multiple pathogenic and toxic species in complex matrices. Sandwich fluoroimmunoassays are performed on the surface of a patterned microscope slide that acts as an optical waveguide. Fluorescence from immunocomplexes formed on the slide surface is excited using the evanescent field, an electromagnetic component of light, and the pattern of fluorescence is imaged using a charge-coupled device camera. Using the evanescent wave for excitation allows real-time imaging. Alternatively, a confocal scanner can also be used to detect and quantify fluorescent spots. A method for immobilizing capture antibodies, performing assays, and detecting bound targets is presented.
- 平板菌落计数
- 细菌的转导
- 人环孢霉素A(CsA)酶联免疫分析(ELISA)
- 鳗鲡
- Loop-Mediated Isothermal Amplification-Based Detection of Fusarium graminearum
- Purification and Characterization of Mast Cell Tryptase and Chymase from Human Tissues
- Use of Genome-Scale Metabolic Models in Evolutionary Systems Biology
- Nutritional Status of Grape Juice
- NMR Characterization of Amphipathic Helical Peptides
- Protein Turnover Methods in Single-Celled Organisms: Dynamic SILAC