DNA Extraction from Natural Environments
Until recently, studies on microbial communities present in natural environments relied on conventional optical microscopic observation and cultivation-based approaches. Although these traditional approaches remain valuable, they have a number of limitations. The most commonly cited limitation stems from the finding that the majority of microbial cells in natural environments cannot be cultured in the laboratory—the phenomenon of nonculturability. The fraction of bacteria in soil, e.g., which can be cultured forms only approx 0.3% of the total number of cells that are observed microscopically (1 ). By contrast, newer methods based on the use of molecular biology methods to analyze total extracted DNA from natural specimens, potentially sample the entire population and, thus, provide a better representative picture of the total microbial community.
- Production of T-Cell Lines
- Evaluation of the Insecticidal Efficacy of Wild-Type and Recombinant Baculoviruses
- Streptomyces:Protocols/Mini-Maxi Prep
- 类毒素
- 猪细小病毒抗体(RPV)酶联免疫分析
- Adenovirus Capsid Chimeras: Fiber Terminal Exon Insertions/Gene Replacements in the Major Late Transcription Unit
- Chromatin Immunoprecipitation Analysis in Filamentous Fungi
- Determination of Bacteriophage Genome Size by Pulsed-Field Gel Electrophoresis
- Methods to Determine Proteolytic Activity of Lactic Acid Bacteria
- The Cotton Rat as a Model for Staphylococcus aureus 19 Nasal Colonization in Humans: Cotton Rat S. aureus Nasal Colonization Mod