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实验基础>Expression and Purification of Viral Glycoproteins Using Recombinant Vaccinia Viruses for Functional and Structural Studies
Expression and Purification of Viral Glycoproteins Using Recombinant Vaccinia Viruses for Functional and Structural Studies
关键词: expression purification来源: 互联网
Methods for generating recombinant vaccinia viruses for the expression of foreign viral glycoproteins in mammalian cell lines and the purification of expressed viral glycoproteins are described. These methods are based on many years of experience with the influenza hemagglutinin glycoprotein (HA). However, they are applicable for studies on other viral glycoproteins, and with slight modifications, could be useful for cellular proteins as well.
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