实验方法>
微生物学技术>
实验基础>Molecular Tools for Carotenogenesis Analysis in the Zygomycete Mucor circinelloides
Molecular Tools for Carotenogenesis Analysis in the Zygomycete Mucor circinelloides
关键词: molecular tools carotenogenesis来源: 互联网
The carotene producer fungus Mucor circinelloides is the zygomycete more amenable to genetic manipulations by using molecular tools. Since the initial development of an effective procedure of genetic transformation, more than two decades ago, the availability of new molecular approaches such as gene replacement techniques and gene expression inactivation by RNA silencing, in addition to the sequencing of its genome, has made Mucor a valuable organism for the study of a number of processes. Here we describe in detail the main techniques and methods currently used to manipulate M. circinelloides , including transformation, gene replacement, gene silencing, RNAi, and immunoprecipitation.
推荐方法
- 肺炎双球菌(Dneumococcus)
- Surface Plasmon Resonance Genosensor for the Detection of Fusarium culmorum
- Capillary Electrophoresis Chips for Fingerprinting Endotoxin Chemotypes from Whole-Cell Lysates
- Baculovirus Transfer Vectors
- Clinical, Epidemiologic, and Laboratory Aspects of Methicillin-Resistant Staphylococcus aureus Infections
- Animal Model for In Vivo Evaluation of Cholesterol Reduction by Lactic Acid Bacteria
- Using MCL to Extract Clusters from Networks
- The Chemical Identification and Analysis of Aspergillus nidulans Secondary Metabolites
- Transmission Electron Microscopy and Immunogold Staining of Mollicute Surface Antigens
- BAC Library Construction