Titration of Reagents
This chapter examines in detail the necessary practical skills needed to facilitate the development and sustainability of ELISAs. Basic methodologies to set up all systems are discussed so that the reader can both investigate the possibilities of tests using his or her reagents and use with confidence the reagents obtained from other sources. As with all other tests, it is vital that scientists realize the principles of the methods to allow good judgement based on quantifiable and controllable features. In other words, there should be a full understanding of what is being performed in the laboratory. The intention of this training is to produce new skills and understanding as well as to encourage critical assessment. Such a mentality is necessary and supported through the application of statistical criteria to results and to continuous monitoring of performance.
- 微观世界的窗口:研究微生物在多孔介质中如何运输的简便微流体系统
- 微生物学实验须知
- 细菌的菌落特征与繁殖
- Quantitative Detection of Aspergillus spp. by Real-Time Nucleic Acid Sequence-Based Amplification
- Phage Host Range and Efficiency of Plating
- Selecting Antibodies to Cell-Surface Antigens Using Magnetic Sorting Techniques
- Assessment of Magnaporthe grisea mating type by spore PCR
- Baculovirus Insecticide Production in Insect Larvae
- Meat Protein Degradation by Tissue and Lactic Acid Bacteria Enzymes
- Microbial Community Profiling Using Terminal Restriction Fragment Length Polymorphism (T-RFLP) and Denaturing Gradient Gel Elect