Development of Lepidopteran Cell Lines
Ever since the early reports of Goldschmidt (1 ) and Trager (2 ) on the in vitro cultivation of lepidopteran tissues, researchers have been interested in using lepidopteran cell cultures for investigations in insect pathology, genetics, and physiology. With the successful development of continuous cell lines by Gaw et al. (3 ) and Grace (4 ), interest in the use of lepidopteran cell cultures increased dramatically (5 –9 ). As a result of this increased interest, over 150 lepidopteran continuous cell lines were reported by 1991 (10 –4 ), and several additional cell lines have been developed from lepidoptera since then (15 – 17 , Sohi unpublished data). With the recent advances in biotechnology and the development of baculovirus expression vectors, lepidopteran cell cultures have become invaluable for basic biological and biotechnological research (18 , 19 ).
- MFC培养基
- 明胶磷酸盐缓冲液
- Isolation, Flow Cytometric Analysis, and Suppression Assay of CD4+ CD25+ T-Regulatory Cells
- Rapid Methods for the Extraction and Archiving of Molecular Grade Fungal Genomic DNA
- Nucleotide Excision Repair Coupled to Chromatin Assembly
- The FERMEX Method for Metabolite-Enriched Fungal Extracts
- Mixed Infections with Rotaviruses: Protocols for Reassortment, Complementation, and Other Assays
- An SDS-PAGE-Based Enzyme Activity Assay for the Detection and Identification of Histone Acetyltransferases
- Herpesviruses
- Electroporation of Physarum polycephalum