PCR Genotyping from the Embryo Yoke Sac
This method is modified from Dr. Lyn Corcoran (WEHI)
Disclaimer: The following set of protocols were contributed by various members of our lab (past and present): Christine Andrews, Fiona Christensen, Neil Della, Ross Dickins, Debbie Donald, Andrew Holloway, Gary Hime, Colin House, Yinling Hu, Rachael Parkinson, Nadia Traficante, Hannah Robertson, Ping Fu and Dennis Wang. Special thanks to Vicki Hammond, Frank Kontgen and Maria Murphy, who contributed many of the ES cell protocols. Sections dealing with Photomicroscopy, Polyclonal and Monoclonal Antibody Production were provided by members of Gerry Rubin's Laboratory (Berkeley). Any comments in the methods (technical errors etc.) E-mail: d.bowtell@pmci.unimelb.edu.au David Bowtell PMCI October 1998
- 双荧光素酶报告基因测试
- Transgenic Animals(图)
- 转基因小鼠制备protocol
- DNA ultrapurification
- Preparation of Microinjection Buffer
- Protocol for isolating High Molecular Weight DNA from yolk sacs of 9.5 dpc or later embryos
- Protocol for isolating DNA from embryo yolk sacs, or toes, or 2 mm tail for PCR
- Blastocyst embryo transfer into pseudopregnant recipient female mouse(图)
- Wholemount Immuno-flurescent
- AP Assay and FGF Binding