Immunochemical Detection of UV-Induced DNA Damage and Repair
关键词: immunochemical detection来源: 互联网
Because of a substantial rise in the incidence of skin cancer in the United Kingdom and elsewhere a greater awareness of the role of sun-induced cutaneous genetic damage has developed. This, in turn, has increased interest in the cellular mechanisms responsible for tumorigenesis, and the need to develop experimental methodologies to investigate these mechanisms. DNA represents a most important cellular target for ultraviolet radiation (UVR), leading to the formation of various DNA damage products. A number of these products, such as the cyclobutane pyrimidine dimer, have been implicated in the pathogenesis of various UVR-related conditions. In this chapter we detail a number of methods for assessing UVR-induced DNA damage using two antisera which recognize cyclobutane thymine dimers (T-T). Immuno-approaches have a number of benefits over chromatographic techniques, and have been applied herein to quantitatively and qualitatively assess the presence of T-T in cultured keratinocytes, human skin, and urine, providing information about lesion induction and repair.
推荐方法
- Saturation Mutagenesis
- Laboratory Information Management Systems for DNA Barcoding
- The Use of PCR for Differential Screening of cDNA Libraries
- Serial Analysis of Binding Elements for Transcription Factors
- Molecular Analysis of Complement Component C4 Gene Copy Number
- Ultraviolet-Laser Footprinting
- Large-Scale Production of Secreted Proteins in Pichia pastoris
- Prediction of Intrinsic Disorder and Its Use in Functional Proteomics
- Cryopreservation
- Clustering of Gene Expression Data Via Normal Mixture Models