DNA Detection by Cascade Enzymatic Signal Amplification
Although many approaches based on template replication were developed and applied in DNA detection, cross-contamination from amplicons is always a vexing problem. Thus, signal amplification is preferable for DNA detection due to its low risk of cross-contamination from amplicons. Here, we proposed a cascade enzymatic signal amplification (termed as CESA) by coupling Afu flap endonuclease with nicking endonuclease, including three steps: invasive signal amplification, flap ligation, and nicking endonuclease signal amplification. Because of the advantages of low risk of contamination, no sequence requirement of target DNA, and the universal reaction conditions for any target detection, CESA has a great potential in clinical diagnosis.
- S1 Nuclease Protection Mapping
- GCG: Translation of DNA Sequence
- Agarose and Polyacrylamide Gel Electrophoresis
- A Rapid PCR-Based Colony Screening Protocol for Cloned Inserts
- Developing Extrachromosomal Gene Expression Vector Technologies: An Overview
- Methylation Analysis by DNA Immunoprecipitation (MeDIP)
- In Vitro Precursor MicroRNA Processing Assays Using Drosophila Schneider-2 Cell Lysates
- Development and Adaptation of the PRINS Technology: An Overview
- DNA Copy Number Data Analysis Using the CGHAnalyzer Software Suite
- Expression Pattern Analysis of MicroRNAs in Caenorhabditis elegans