Sending Sera to Testing for Mouse Pathogens
关键词: Transgene Mice Embryo Transfer来源: 互联网
Bleed mice: Obtain 300-400µl from mice of various ages (older mice that have been in the room for an extended period) and strains (include nude mice if in the room). 5-8 mice is a good number.
Allow blood to clot and clot to retract o/n and collect serum next day. Avoid hemolysis.
Send sera with an ice pack to:
Dr. Bob Stevenson
Institute of Medical and Veterinary Science
Frome Rd
Adelaide 5000
Phone 08 228 7377
Testing routinuely includes: - Rotavirus - MHV-RCV - Mycoplasma - PVM - Sendi virus - GD7 - LCM
Cost is ca. $6 per test.
推荐方法
- 动物转基因技术的常用方法
- 转基因小鼠制备实验
- Gene Knockout Techniques(1) The Knockout Process
- LacZ Cell Staining and β-galactosidase Embryo Staining
- Preparation of Microinjection Buffer
- Combined LacZ staining and in situ hybridization
- Vasectomy of Male Mice
- Wholemount Immuno-flurescent
- Isolation of linearized plasmid DNA constructs for microinjection to produce transgenic animals
- Isolation of linearized BAC DNA for microinjection to produce transgenic animals