Assaying Drug-Induced Apoptosis
Apoptosis, frequently referred to as “programmed cell death” or “cell suicide,” is an active and physiological mode of cell death, where the cell itself prepares and executes the program of its own demise. A complex, multistep mechanism regulates the cell’s response to various stimuli by apoptosis (reviewed in refs. 1 ,2 ). The regulatory system involves the presence of at least two distinct checkpoints, one controlled by bcl-2/bax family of proteins (3 ), and another by cysteine and possibly serine proteases (4 ). There is a close association between apoptosis and regulation of cell proliferation as well as DNA repair.
- Mass Spectrometry for Protein Quantification in Biomarker Discovery
- DNase i footprinting using PCR-generated end-labeled DNA probes
- AlleleID: A Pathogen Detection and Identification System
- Microarray Analysis Using RNA Arbitrarily Primed PCR
- Agarose Gel Electrophoresis and Polyacrylamide Gel Electrophoresis for Visualization of Simple Sequence Repeats
- Bioinformatics Identification of Coevolving Residues
- Construction and Analysis of Full-Length and Normalized cDNA Libraries from Citrus
- Surgical Techniques for the Generation of Mutant Mice
- Electrophoresis of Sequence Reaction Samples
- Transfection Technologies