Highly Effective Delivery of Foreign DNA to Adherent Cells via Polybrene/DMSO-Assisted Gene Transfer
The ability to transfect cells with native genometic DNA and engineered gene/vector constructs has played a leading role in accelerating our understanding of gene regulation and function. Yet, despite significant diversification of gene delivery strategies, efforts to expand the repertory of cell types amenable to DNA-mediated gene transfer continue to stumble over a recurrent obstacle—namely, the sensitivity that recipient cells often manifest towards the gene transfer procedure itself (1 ,2 ). To minimize the likelihood of cytopathic effects engendered by chemical and physical methods investigators, more and more, are adopting low toxicity synthetic polymers and ligand-mediated strategies to dispatch copious amounts of exogenous DNA to the surfaces of target cells (3 –10 ).
- Assessing DNA Structures with 125I Radioprobing
- cDNA Libraries from a Low Amount of Cells
- Simultaneous In Situ Detection of RNA, DNA, and Protein Using Tyramide-Coupled Immunofluorescence
- Sequencing Bacterial Artificial Chromosomes
- Evaluating the Quality of Data From Microarray Measurements
- Generation of Random Fragments by Sonication
- Simultaneous Labeling of Single- and Double-Strand DNA Breaks by DNA Breakage Detection-FISH (DBD-FISH)
- A Bacterial/Yeast Merged Two-Hybrid System: Protocol for Bacterial Screening
- eQTL Analysis in Mice and Rats
- Recovery of DNA for PCR Amplification from Blood and Forensic Samples Using a Chelating Resin