Multicolor FISH
Chromosome “painting” by fluorescent labeled probes for each human chromosome has been available since the late 1980s. The DNA or RNA radioactive isotopes have been in use since 1969 as nucleic acid probes (1 ,2 ). The introduction of fluorescent labeled probes by Bauman et al. (1980) presented an attractive alternative for chromosomal in situ hybridization (3 ). Based on unique DNA sequences specific to individual chromosomes, painting probes were generated for each human chromosome (4 ,5 ). Pinkel et al. (1988) used in situ hybridization of 120 fluorescent DNA probes (FISH) along the entire length of chromosome 4 to generate a specific painting probe for this chromosome (6 ). By the early 1990s, whole chromosome painting probes (wcp) were commercially available for each human chromosome in two colors (green and orange). Jalal et al. presented the characteristics of the direct labeled wcps for every chromosome and their utility in clinical practice (7 ). The ability to resolve chromosomal anomalies was greatly enhanced when wcps became available as a complement to chromosome analysis by banding techniques.
- Generation of Cre Recombinase-Expressing Transgenic Mice Using Bacterial Artificial Chromosomes
- FISH for Pre-implantation Genetic Diagnosis
- Production of 35S-Labeled Proteins in E. coli and Their Use as Molecular Probes
- Construction and Use of Cosmid Contigs
- Introduction to the Analysis of Environmental Sequences: Metagenomics with MEGAN
- RNase Protection Assay for Quantifying Gene Expression Levels
- BAC Transgenes, DNA Purification, and Transgenic Mouse Production
- Applications of Array-CGH for Lung Cancer
- Use of Real-Time Polymerase Chain Reaction for the Detection of Fetal Aneuploidies
- Delivery of DNA to Lung Airway Epithelium