Mutation Detection by Southern Blotting
Following the discovery of the structure of DNA in 1953, it became clear that scientists needed to be able to distinguish different DNA sequences. In 1975, Edward Southern published details of a new method for detecting DNA fragments based upon their specific sequence. An indication of the importance of his work is that the technique was eponymously named after him and that subsequent methods based loosely on similar principles were named using a play on his surname (western and northern blot). The simplicity and effectiveness of the technique led to its universal acceptance as a standard method for identification of DNA sequences. In the modern laboratory where turn-around times assume ever greater importance, the process can seem relatively time-consuming. In some cases, this has led to its replacement by more rapid techniques such as long-range PCR. Nevertheless, more than 30 years after its invention, the Southern blot remains a cornerstone of molecular biology.
- Generation of scFv from a Phage Display Mini-Library Derived from Tumor-Infiltrating B-Cells
- Semi-synthesis of Glycoproteins from E. coliThrough Native Chemical Ligation
- Minigene-Containing Retroviral Vectors Using an Alphavirus/Retrovirus Hybrid Vector System: Production and Use
- Inverse PCR: cDNA Cloning
- Detection of Enterococci in Freshwater and Seawater (16S and 23S rRNA Enterococcus Oligonucleotide Probes)
- Removal of Interfering Substances in Samples Prepared for Two-Dimensional (2-D) Electrophoresis
- Design and Construction of Multigenic Constructs for Plant Biotechnology Using the GoldenBraid Cloning Strategy
- Retroviral Modification of Mesenchymal Stem Cells for Gene Therapy of Hemophilia
- Characterization of Structural Determinants of Type 1 Corticotropin Releasing Hormone (CRH) Receptor Signalling Properties
- Nonfamilial Breast Cancer Subtypes