σ-Like plastid transcription factors
关键词: transcription factors来源: 互联网
Work with chloroplast RNA polymerase from various plant species has established the presence of transcription factors that either act on the DNA template or on other polypeptides of the transcription complex. The first example was a 26-kDa polypeptide from maize chloroplasts, referred to as S-factor (1 ), which was shown to stimulate the rate of transcription from chimeric supercoiled templates. In its presence, maize chloroplast RNA polymerase was found to transcribe chloroplast sequences preferentially as compared to vector sequences, if the plasmid template was in a supercoiled form. The S-factor appears to act generally, rather than on a specific set of chloroplast genes (2 ). Another factor described is a 115-kDa DNA-binding protein from pea chloroplasts, which was shown to interact with a 5′ region of a specific chloroplast gene (3 ). Further examples include a DNA-binding polypeptide of approx 70 kDa found associated with partially purified RNA polymerase from spinach chloroplasts (4 ) and several DNA-binding polypeptides that copurified with chloroplast RNA polymerase transcription complexes from mustard (5 ) and tobacco (6 ).
推荐方法
- Using the SELEX Combinatorial Chemistry Process to Find High Affinity Nucleic Acid Ligands to Target Molecules
- A Microtiter-Plate-Based High Throughput PCR Product Purification Method
- Mini-Mu Transposon Mutagenesis of Ethanologenic Zymomonas mobilis
- Application of Gene Ontology to Gene Identification
- Analysis of Sperm Aneuploidy by PRINS
- Avoiding False Discoveries in Association Studies
- Biomedical and Agricultural Applications of Animal Transgenesis
- Next-Generation Sequencing of the Human Olfactory Receptors
- RT-PCR-Based Approaches to Generate Probes for mRNA Detection by In Situ Hybridization
- Chromosome Structure and Homologous Chromosome Association During Meiotic Prophase in Caenorhabditis<br> eleg