Reconstitution of Chromatin In Vitro
It is now generally believed that DNA methylation is responsible for genomic imprinting in mammals (1 ). Recent experimental evidence has provided an elegant mechanism for repression of gene expression by DNA methylation. This evidence suggests that proteins that recognize specifically methylated CpGs may contribute to the formation of inactive chromatin (2 -5 ). Nucleosomes are the basic unit of chromatin, consisting of a core of 146 bp of DNA wrapped around a histone octamer (two molecules of each of H2A, H2B, H3, and H4) and a stretch of linker DNA between adjacent nucleosomes. The binding of a fifth histone, known as a linker histone or H1, promotes the packaging of strings of nucleosomes into a 30 nm chromatin fiber (6 ). Packaging of DNA into nucleosomes and the chromatin fiber greatly restricts the availability of the DNA for nuclear processes such as transcription.
- Next-Generation Sequencing of miRNAs with Roche 454 GS-FLX Technology: Steps for a Successful Application
- Structure Analysis of MicroRNA Precursors
- Inverse PCR: cDNA Cloning
- Increasing the Average Abundance of Low-Abundance cDNAs by Ordered Subdivision of cDNA Populations
- Construction of microRNA-Containing Vectors for Expression in Mammalian Cells
- Cytokine Gene Polymorphisms: Methods of Detection and Biological Significance
- Generation of Full-Length cDNA Libraries: Focus on Plants
- Fluorescent Site-Specific Labeling of Escherichia coli Expressed Proteins with Sfp Phosphopantetheinyl Transferase
- Building Block Synthesis Using the Polymerase Chain Assembly Method
- Pyrosequencing Applications