Se-Derivatized RNAs for X-ray Crystallography
关键词: se-derivatized se来源: 互联网
Selenium derivatization of RNA is an important strategy for crystal structure determination and functional studies of noncoding RNAs and protein–RNA interactions. We describe here the synthesis of nucleoside 5′-(α-P-seleno)-triphosphate analogs (Se-NTPs) and their use in vitro transcription and purification of Se-derivatized RNA samples (phosphoroselenoate RNA, PSe-RNA).
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