Isolation of RNA and DNA from Single Preimplantation Embryos and a Small Number of Mammalian Oocytes for Imprinting Studies
Researchers whose experimental models are mammalian oocytes and preimplantation embryos are often limited by the yield of nucleic acids that can be isolated from such a small sample size. In addition, the limited number of cells from these types of samples makes the simultaneous recovery of RNA and DNA very difficult and often sample pooling is necessary to increase nucleic acid yield. Here we report a simple set of procedures using commercially available kits that results in consistent yield and quality of nucleic acids. After sample lysis, RNA is isolated and converted to a reusable cDNA library. Following RNA isolation, DNA is precipitated, isolated, and bisulfite converted for DNA methylation studies. Our results demonstrate the feasibility of isolating RNA and DNA from a small number of cells with repeatability of results.
- Design of Tag SNP Whole Genome Genotyping Arrays
- Laboratory Information Management Systems for DNA Barcoding
- Coupling of DNA Helicase Function to DNA Strand Exchange Activity
- Single Marker Family-Based Association Analysis Not Conditional on Parental Information
- Combining ES Cells with Embryos
- Two-Dimensional Crystallization of Soluble Protein Complexes
- Vector Systems for Prenatal Gene Therapy: Choosing Vectors for Different Applications
- Extraction of DNA from Paleofeces
- Probability, Statistics, and Computational Science
- In Situ DNA Ligation as a Method for Labeling Apoptotic Cells in Tissue Sections: An Overview