热门搜索词：
Quantification of Methylated DNA by HeavyMethyl Duplex PCR
关键词： quantification methylated dna来源： 互联网
The HeavyMethyl (HM) assay is a real-time PCR assay suitable for the qualitative and quantitative DNA methylation analysis of fresh, frozen, or formalin-fixed paraffin-embedded tissues and remote samples, such as serum, plasma, and urine. The HM uses a methylation-specific oligonucleotide blocker and a methylation-specific probe to achieve methylation-specific amplification and detection. A protocol for a duplex real-time PCR for the analysis of the methylation status of the GSTP1 exon1 in prostate tissue samples is presented. This chapter describes the preparation and analysis of a combined HM GSTP1 Exon1 and GSTP1 reference assay.
推荐方法
- Typing of Y Chromosome SNPs With Multiplex PCR Methods
- Fast and Accurate Method to Purify Small Noncoding RNAs from Drosophila Ovaries
- The Reference Point Method in Primer Design
- Pyrosequencing Genotype Storage Techniques
- RNA Visualization in Bacteria by Fluorescence In Situ Hybridization
- Antitumor Vaccination with Synthetic mRNA: Strategies for In Vitro and In Vivo Preclinical Studies
- Separation of PCR Fragments by Means of Direct Blotting Electrophoresis
- Molecular Analysis of Mitochondrial DNA Point Mutations by Polymerase Chain Reaction
- Development and Adaptation of the PRINS Technology: An Overview
- Multiplex Amplification Refractory Mutation System for the Detection of Prothrombotic Polymorphisms
移动版：