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Inducible Gene Expression in Mammalian Cells and Mice
Inducible expression of desired transgenes in mammalian cells and animals is a current priority in basic and applied research, biopharmaceutical manufacturing, gene therapy, and tissue engineering, as well as in drug discovery. Among the most prominent human-compatible transgene control technologies are engineered promoter/transactivator configurations that adjust heterologous target gene transcription in response to clinically licensed antibiotics (tetracyclines, streptogramins, macrolides). In this chapter we provide a detailed case study on macrolide-inducible expression of the human model glycoprotein SEAP (human placental secreted alkaline phosphatase) in transgenic Chinese hamster ovary (CHO) cell cultures or following implantation of microencapsulated CHO cells into mice.
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- Oligonucleotide Array Comparative Genomic Hybridization
- High-Throughput T-Cell Epitope Discovery Through MHC Peptide Exchange
- PRINS Evaluation of Chromosome Instability in Mammalian Cells by Detection of Repetitive DNA Sequences in Micronuclei
- Introduction to Vectors for Suicide Gene Therapy
- A Short Primer on the Functional Analysis of Copy Number Variation for Biomedical Scientists
- In Vivo Analysis of RNA Editing in Plastids
- Engineered Mammalian Chromosomes in Cellular Protein Production: Future Prospects
- Visualizing Olfactory Receptor Expression and Localization in Drosophila
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