Chromosome Banding and Identification: Fluorescence
General characteristics of chromosome banding and banding techniques have been described in the previous chapter (Chapter 6 ). In general, fluorescence banding provides much the same information as banding using absorption staining, but fluorescence staining methods are generally simpler than banding methods using absorption staining and, in many respects, are more reliable. On the other hand, fluorescent preparations are not permanent and have to be examined using a fluorescence microscope. As well as providing the same general information as banding with absorption staining, most fluorescence banding methods also provide specific types of information (for example, on heteromorphisms of heterochromatin) that are not shown by other methods. Details of these applications will be given under the specific methods.
- Selection of Recombinant Antibodies from Antibody Gene Libraries
- Exploring Conservation of Transcription Factor Binding Sites with CONREAL
- Transformation of Yeast Using Bioactive Beads With Surface-Immobilized Yeast Artificial Chromosomes
- MutSDNA Interactions and DNase Protection Analysis with Surface Plasmon Resonance
- Development of an In Vitro mRNA Decay System in Insect Cells
- A Post-Labeling Approach for the Characterization and Quantification of RNA Modifications Based on Site-Directed Cleavage by DNA
- High Throughput Whole Mount In Situ Hybridization of Zebrafish Embryos for Analysis of Tissue-Specific Gene Expression Changes A
- Quality Control in PCR
- In Situ DNA Ligation as a Method for Labeling Apoptotic Cells in Tissue Sections: An Overview
- Characterization of Constitutional Chromosome Abnormalities by Comparative Genomic Hybridization