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基因表达差异显示实验>A Nonviral Cytoplasmic T7 Autogene System and Its Applications in DNA Vaccination
A Nonviral Cytoplasmic T7 Autogene System and Its Applications in DNA Vaccination
关键词: applications dna vaccination来源: 互联网
The use of DNA vectors to elicit an immune response has produced a lot of interest. Unfortunately, one of the limiting factors has been the problem of gene expression. In order to obtain a strong expression of the vaccinating gene, several steps are necessary. The vector has to be delivered in such a way that it is not being degraded by the immune nor by the hepatic system; it has also to enter efficiently the targeted cells; and it must be expressed in the appropriate compartment of the cells at a high level. For these reasons, we have developed a gene expression vector that contains a T7 autogene and is being expressed in the cytoplasm of the cells (1 ,2 ). We will describe this system and two possible applications: infectious disease vaccination and tumor ablation. The latter application may be combined with DNA vaccination against cancer cells.
推荐方法
- 基因差异表达技术——差别杂交与扣除杂交
- Dendrimer-Mediated Cell Transfection in Vitro
- Chromosomal Expression of Foreign and Native Genes From Regulatable Promoters in Escherichia coli
- Rat Spermatogonial Stem Cell-Mediated Gene Transfer
- Direct Analysis of Chromosome Methylation
- Isolation of Target Gene Promoter/Enhancer Sequences by Whole Genome PCR Method
- Identification and Quantification of Differentially Methylated Loci by the Pyrosequencing™ Technology
- The Detection of Latent Virus Infection by Polymerase Chain Reaction
- Sequencing by Hybridization Arrays
- Prenatal Detection of Chromosome Aneuploidy by Quantitative Fluorescence-PCR