实验方法>
遗传学实验技术>
基因表达差异显示实验>Resolution of DNA Molecules by One-Dimensional Agarose-Gel Electrophoresis
Resolution of DNA Molecules by One-Dimensional Agarose-Gel Electrophoresis
关键词: resolution dna molecules来源: 互联网
Agarose-gel electrophoresis is used to separate DNA molecules on the basis of size and shape (1 -4 ). Since DNA is negatively charged, the charge-to-mass ratio is constant. Thus, migration through agarose is inversely proportional to the size of the molecule. However, the electrophoretic mobility of DNA in agarose is also affected by the shape of the DNA, the pore size of the matrix (agarose concentration), temperature, the ionic strength of the electrophoresis buffer, the applied voltage/field strength, and the presence of intercalators (reviewed in 5 ,6 ).
推荐方法
- GCG: Production of Multiple Sequence Alignment
- Assessing DNA Structures with 125I Radioprobing
- Identification of Sequence-Specific DNA-Binding Proteins by Southwestern Blotting
- Using the Gibbs Motif Sampler for Phylogenetic Footprinting
- Antisense Oligonucleotides: Design, Construction, and Applications to Cardiac Allograft Transfer
- Ancient DNA Extraction from Plants
- Markers of Poly (ADP-Ribose) Polymerase Activity as Correlates of DNA Damage
- Genomic Sequencing
- Three-Dimensional Reconstruction of Trypanosoma brucei Editosomes Using Single-Particle Electron Microscopy
- Applying UV Crosslinking to Study RNAProtein Interactions in Multicomponent Ribonucleoprotein Complexes