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Calcium Phosphate Mediated Gene Transfer into Established Cell Lines
关键词： calcium phosphate mediated gene来源： 互联网
DNA transfection is one of the most important techniques in molecular genetics. It is this technique that has made possible the dissection of complex eukaryotic genes and the characterization of the function of their components (1 –7 ) as well as the isolation of particular genes on the basis of their expression in cultured cells (8 –11 ).
推荐方法
- Expressed Sequence Tags: Medium-Throughput Protocols
- High-Throughput Expression Screening and Purification of Recombinant Proteins in E. coli
- Reporter Molecules in Genetically Engineered Mice
- Aggregation Effect in Microarray Data Analysis
- Staggered Extension Process (StEP) In Vitro Recombination
- Immunostaining of Squash Preparations of Chromosomes of Larval Brains
- Analysis of Survivin-Specific T Cells in Breast Cancer Patients Using Human DCs Engineered with Survivin mRNA
- DNA-Binding studies using in vitro synthesized myb proteins
- Retroviral-Mediated Gene Therapy for the Differentiation of Primary Cells into a Mineralizing Osteoblastic Phenotype
- Rapid and Nonradioactive Screening of Recombinant Libraries by PCR
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