Two-Dimensional Agarose Gel Electrophoresis of DNA Topoisomers
The electrophoretic velocity of a duplex DNA ring is mainly determined by its overall shape. Consequently, DNA topoisomers of opposite supercoiling handedness can have identical gel velocity, and topoisomers highly supercoiled cannot be separated beyond some point. These problems are overcome by two-dimensional agarose gel electrophoresis, which involves two successive electrophoresis steps in one gel slab. The first and second electrophoresis steps are conducted in orthogonal directions with different concentrations of DNA intercalating agents. These compounds alter the overall shape of the DNA and, thereby, change the relative mobility of individual DNA topoisomers.
- Poly(T) Adaptor RT-PCR
- Bar-Coded, Multiplexed Sequencing of Targeted DNA Regions Using the Illumina Genome Analyzer
- Histone H2AX Phosphorylation: A Marker for DNA Damage
- Generation of cDNA Libraries from RNP-Derived Regulatory Noncoding RNAs
- Minisatellites and MVR-PCR for the Individual Identification of Parasite Isolates
- Studying Repair of a Single Protein-Bound Nick In Vivo Using the Flp-Nick System
- Statistical Methods for Building Random Transposon Mutagenesis Libraries
- Epitope Mapping by Region-Specified PCR-Mutagenesis
- Inducible and Conditional Promoter Systems to Generate Transgenic Animals
- DNA Microarrays: History and Overview