Combination Suicide Gene Therapy
关键词: combination suicide gene来源: 互联网
Suicide genes (SG) can be viewed as negatively selectable marker genes. Their gene products convert otherwise nontoxic prodrugs (PD) into cytotoxic metabolites. The enzymatic conversion leads to high levels of the activated toxic drug within genetically modified cells and ultimately kills the cells. Several studies have shown that when SG-expressing tumor cells are mixed with parental, nongenetically modified cells, prodrug treatment induces toxic effects in the nonmodified cells. This phenomenon has been termed “bystander (killing) effect.”
推荐方法
- DNA-Methylation Analysis by the Bisulfite-Assisted Genomic Sequencing Method
- Software for Genetic Linkage Analysis
- Overprint Immunoassay Using Protein A Microarrays
- Biolistic Transformation of Caenorhabditis elegans
- Gene Delivery to Mammalian Cells by Microinjection
- Colony Management
- Retinal Tissue
- BAC Transgenes, DNA Purification, and Transgenic Mouse Production
- Spinal Muscular Atrophy
- Isolation of Kinetoplast DNA