Family-Based Linkage Disequilibrium Tests Using General Pedigrees
Linkage disequilibrium (LD) mapping has been established as a promising approach to identifying disease genes. The presence of a disease gene located near a marker locus may cause LD between the marker and the disease loci. In LD mapping, we assume that some of the affected individuals may have a common ancestor carrying the mutation and that mutation carriers are likely to share alleles at the markers loci close to the disease gene. This chapter reviews the concept of LD mapping and outlines the advantages and disadvantages of two LD mapping approaches capable of handling general pedigrees: the family-based association test (FBAT) and pseudomarker. In summary, the pseudomarker statistical approach and the FBAT approach are both expected to offer reasonable statistical power to detect genes underlying complex traits. However, when the pedigree structure is more complicated, or when the number of informative families is limited, the pseudomarker approach is anticipated to outperform FBAT.
- FRET-Based Real-Time DNA Microarrays
- Seamless Ligation Cloning Extract (SLiCE) Cloning Method
- SI Mapping Using Single-Stranded DNA Probes
- The Use of Double-Stranded RNA to Knock Down Specific Gene Activity
- A Neutral Glyoxal Gel Electrophoresis Method for the Detection and Semi-quantitation of DNA Single-Strand Breaks
- Ancient Gene Transfer as a Tool in Phylogenetic Reconstruction
- Genetic Variation Analysis for Biomedical Researchers: A Primer
- Measuring DNA Content by Flow Cytometry in Fission Yeast
- Protein Antigen Delivery by Gene Gun-Mediated Epidermal Antigen Incorporation (EAI)
- Protein Identification by Mass Spectrometric Analyses of Peptides